基于高效液相色谱法的小麦中呕吐毒素检测方法优化研究OA

This study aimed to optimize the method for determining deoxynivalenol in wheat using high-performance liquid chromatography,improving the accuracy and efficiency of detection.By investigating the effects of different mobile phase ratios,extraction solvents,and extraction methods on the deoxynivalenol content in wheat,an analytical method based on immunoaffinity column purification and high-performance liquid chromatography with high accuracy and good reproducibility was established.The results showed that the chromatographic peak area was linearly related to the concentration of deoxynivalenol,with a detection limit of 0.083 μg/kg and recovery rates of 94.05％~99.16％.When spiked at 1.0 μg/mL,the relative standard deviation(RSD)was 0.37％.This method met the national standard requirements for deoxynivalenol detection and provided more reliable technical support for grain quality and safety testing.