罗汉果提取物中农药残留脱除工艺研究OA

Monk fruit(Luo Han Guo)extract is often contaminated with various pesticide residues,including acetamiprid,clothianidin,imidacloprid,thiamethoxam,and propamocarb during industrial production,this study systematically investigated a synergistic process for the simultaneous removal of multiple pesticide residues from monk fruit extract.Using dynamic adsorption mode,the adsorption and separation behaviors of conventional macroporous adsorption resins,specific pesticide-removing resins,and cation exchange resins toward the extract,mogroside V(MV),and pesticide residues were examined.The effects of different washing solutions,ethanol elution gradients and concentrations,and loading volumes on pesticide removal efficiency and MV recovery were also evaluated.The results indicated that the optimal process for removing multiple pesticide residues consists of the following steps:adsorption of the concentrated juice of monk fruit onto macroporous adsorption resin,elution with ethanol and concentration of the aqueous phase,and subsequent treatment with a tandem column system combining a specific pesticide-removing resin and a cation exchange resin.After this treatment,the contents of acetamiprid,clothianidin,imidacloprid,thiamethoxam,and propamocarb in the extract were all reduced to below 10 μg/kg,while the recovery of MV remained above 90%.Furthermore,the results showed that simply selecting specific types of adsorption resins,using acid/base washing,or applying gradient ethanol elution could not achieve precise removal of multiple pesticide residues.Among the eight specific pesticide-removing resins evaluated,LKS11-001×7 exhibited the best overall performance in terms of pesticide removal rate,MV recovery,and removal precision.After treatment with the LKS11-001×7 tandem column system,the contents of all five pesticide residues in the extract were below 10 μg/kg.Under this control standard,the processing capacity of LKS11 resin for monk fruit extract reached(1007.21±13.83)g/L.