苜蓿来源叶绿素铜的超临界CO2萃取工艺优化OA

Chlorophyll copper serves as a widely used natural green pigment in the food industry,making quality improvement through purification processes critically important.This study aimed to develop a green and efficient supercritical CO2 extraction and purification method focusing on enhancing product quality of crude chlorophyll copper derived from alfalfa.Using chlorophyll copper content and extraction yield as indicators,a multi-index evaluation was conducted by the comprehensive scoring method(with a weight of 0.6 for content and 0.4 for yield).Through single-factor experiments and orthogonal experimental design,the effects of extraction pressure,temperature,CO2 flow rate,and time on the extraction efficiency were systematically investigated.The optimal process parameters were determined as follows:extraction pressure of 21 MPa,extraction temperature of 55℃,CO2 flow rate of 20.0 L/h,and extraction time of 3.0 h.Under these optimized conditions,the average content of chlorophyll copper reached 23.87％,representing a 77.34％increase compared to the raw material(13.46％),and the average extraction yield was 90.53％.The refined product effectively eliminated the distinctive odor of alfalfa,and its color was significantly improved,exhibiting a bright and pure dark green hue with excellent color stability.This study established a green,efficient,and easily scalable purification process route,providing a technical foundation for the industrial application of chlorophyll copper derived from alfalfa.