高产胆盐水解酶植物乳植杆菌突变株的筛选及其降胆固醇作用研究OA

To obtain a probiotic strain with high bile salt hydrolase(BSH)production,Lactiplantibacillus plantarum WKLPP0040 was subjected to continuous ultraviolet mutagenesis,yielding the mutant strain WKLPP0041.This mutant exhibited significantly enhanced BSH activities toward taurodeoxycholate and glycodeoxycholate,reaching 0.429 and 0.534 μmol/min,respectively,which represented 86.38%and 72.26%increases compared to the parental strain.Furthermore,it demonstrated a high in vitro cholesterol degradation rate of 86%.In a mouse model of hypercholesterolemia,administration of WKLPP0041 significantly reduced serum levels of total cholesterol(TC)and low-density lipoprotein cholesterol(LDL-C),while markedly increasing high-density lipoprotein cholesterol(HDL-C)levels.It also substantially downregulated pro-inflammatory cytokines,including tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6),it significantly increased the abundace of intestinal Bifidobacterium and Lactobacillus,and reduced the abundance of Clostridium perfringens,and improved the gut microbiota composition in mice.These results indicate that WKLPP0041 has potential as a cholesterol-lowering probiotic,and its cholesterol-lowering effect may be associated with enhanced BSH activity and modulation of the gut microbiota.