交链格孢(Alternaria alternata)Gpd1基因的功能分析OA

Alternaria alternata can widely contaminate grains,fruits and vegetables,seriously affecting crop yields and food security.Glycerol is a key energy substance and stress-protective factor in microorganisms,playing an important role in their survival.In the glycerol synthesis metabolic pathway,the glycerol-3-phosphate dehydrogenase encoded by the Gpd1 gene plays a core role and is the rate-limiting enzyme of this metabolic process.This study analyzed the biological function of the Gpd1 gene in Alternaria alternata.Based on the princi-ple of homologous recombination,the knockout mutant ΔGpd1 and its complementation mutant Gpd1-c of Gpd1 in Alternaria alternata were obtained through polyethylene glycol-mediated protoplast transformation method.Phenotypic analysis revealed that compared with the wild type and complementation strain,the growth rate of the knockout mutant ΔGpd1 was reduced,and it showed decreased sensitivity to sodi-um dodecyl sulfate and salt stress,and significantly increased resistance to Congo red.However,there were no significant differences in spore production,tenuazonic acid(TeA)content,expression level of the TeA biosynthesis gene TAS1,pathogenicity to tomatoes,and melanin formation..In conclusion,the Gpd1 gene is involved in the nutritional growth and stress response of Alternaria alternata,but the specific mechanism of action remains to be further studied.