不同产地多花黄精体外抗氧化活性及其组分关联度分析OA

In order to establish ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)chemical fingerprints of Polygonatum cyrtonema from different geographic origins,and to analyze their correlation with in vitro antioxidant activity to screen for potential antioxidant-related quality markers(Q-markers),ethanol extracts of 12 batches of P.cyrtonema collected from Sichuan and Guangxi provinces were prepared.UPLC-Q-TOF-MS was employed to generate chemical fingerprints and evaluate similarity among the batches.Antioxidant capacities were assessed using DPPH and ABTS free radical scavenging assays,and IC50 values were calculated.Grey relational analysis(GRA)and partial least squares regression(PLSR)were combined to determine the correlation between common peaks and antioxidant activity.Key peaks were subsequently identified based on high-resolution MS/MS data.The results showed that significant differences in DPPH and ABTS scavenging capacities were observed among samples from different origins,matching the variations found in fingerprint profiles.Correlation analysis revealed that peaks No.38,28,and 11 strongly correlated with DPPH scavenging activity,while peaks No.51,38,28,and 48 were primarily associated with ABTS scavenging activity.Integrated GRA-PLSR analysis identified peaks No.38,28,11,and 48 as the primary antioxidant-related peaks.Based on MS/MS data,these were tentatively assigned as oleoylethanolamide,p-hydroxy-N-(p-hydroxyphenethyl)cinnamamide,salicylic acid,and genistein,respectively.In conclusion,combining UPLC-Q-TOF-MS fingerprinting with GRA-PLSR correlation analysis effectively bridged the chemical differences in P.cyrtonema with its in vitro antioxidant efficacy.The identified compounds could serve as candidate quality markers,providing a valuable reference for the origin-based quality evaluation and standardization of P.cyrtonema.