维药余甘子牛奶炮制工艺优化及其神经细胞保护作用OA

Abstact:Referencing the traditional uyghur medicine method of soaking Phyllanthus emblica in milk,three batches of emblica decoction pieces were selected.An L9(34)orthogonal test method was used,four factors including milk concentration,material-to-liquid ratio,temperature,and time were investigated by examining the changes in the content of the indicator components gallic acid and vitamin C.This was done to comprehensively screen for the optimal milk-processing technology for emblica.The MTT assay was used to study the pharmacodynamic effects of the processed decoction pieces on the proliferation of PC 12 neural cells.The optimal processing conditions for emblica were determined as follows:100％fresh milk,a material-to-liquid ratio of 1∶5(g/mL),a soaking time of 6 h,and a soaking temperature of 25 ℃.The processed emblica decoction pieces increased the viability of PC 12 cells to 160.06％,showing a significant difference compared to the raw emblica product(P＜0.05).This study standardized the traditional milk-soaking process for emblica,providing a scientific basis for the standardized production and application of emblica decoction pieces.