杜仲、骨碎补、葛根、丹参配伍复方对去卵巢大鼠骨质疏松的改善作用及营养健康应用潜力OA

From the perspective of bone health nutritional intervention and herbal compound product development,to evaluate the effects of different combinations of Eucommia,Drynaria,Pueraria,and Salvia on osteoporosis in ovariectomized rats,as well as their combined regimen with calcium,and to explore their regulatory characteristics on bone metabolism imbalance,providing experimental evidence for the development of related nutritional health products.A postmenopausal osteoporosis rat model was established by ovariectomy(OVX).Female SD rats were randomly divided into a sham operation group,a model group,and seven different herbal compound intervention groups,with 8 rats in each group.After 12 consecutive weeks of oral administration,rats uterine index,wet and dry weight of the tibia,bone mineral density,and bone calcium content were measured;rats tissue morphology was observed using HE staining;and rats serum ALP and TRACP levels were detected by ELISA.The results showed that OVX significantly induced bone metabolism disorder,in the osteoporosis model rats,serum ALP decreased by 55.00％and TRACP increased by 35.20％compared to the sham-operated group(P＜0.05).After compound intervention,herbal combinations such as HM(Eucommia+Drynaria+Pueraria+Salvia)and Ca+HM-H(high-dose calcium+Eucommia+Drynaria+Pueraria+Salvia)significantly improved bone metabolism imbalance.The levels of ALP in HM and Ca+HM-H group rats were approximately doubled,and TRACP decreased by 30.5％compared to the model group(P＜0.05).Additionally,bone mineral density of rats in these two groups increased by 29.4％,and bone calcium content increased by 25.8％;and showing better performance in uterine index recovery(200.00％,P＜0.05)and bone microstructure repair of rats.In conclusion,the herbal compound of Eucommia,Drynaria,Pueraria,and Salvia significantly improved osteoporosis in ovariectomized rats.The full four-herb combination and its combination with calcium were more effective,likely through promoting bone formation,inhibiting bone resorption,and maintaining dynamic bone metabolism balance.Therefore,this compound has potential for further application development into bone health-directed nutritional interventions and functional dietary formulas.