樱桃核醒酒多肽酶解提取工艺的响应面法优化OA
Optimization of enzymatic hydrolysis of cherry stone for preparing anti-hangover peptide by response surface methodology
以废弃樱桃核为原料,提取蛋白粉,优化酶解提取工艺,提取醒酒多肽.以抗氧化能力、水解度和乙醇脱氢酶(ADH)激活作用为评价指标,首先筛选出适宜的蛋白酶种类,继而通过单因素试验明确了酶添加量、料液比及酶解温度对酶解效果影响最为显著,并以此三因素为变量,采用三因素三水平的响应面分析法,以ADH激活率作为目标响应值进行工艺优化.结果表明,最佳酶解条件为加酶量7800 U/g,酶解温度60℃,料液比1∶30(g/mL),在此条件下,樱桃核酶解液的ADH激活率可以达到28.75％,与预测值相近.研究为废弃樱桃核中生物活性多肽的提取提供了重要的理论依据.
In order to optimize the enzymatic hydrolysis process for extracting anti-hangover peptides from discarded cherry stone.Using antioxidant capacity,degree of hydrolysis,and alcohol dehydrogenase(ADH)activation as evaluation indices,a suitable protease was first selected.Single-factor experiments were used to identify that enzyme dosage,solid-liquid ratio,and hydrolysis temperature had the most significant effects on hydrolysis efficiency.These three factors were then used as variables in a three-level,three-factor Box-Behnken design,with ADH activation rate as the response value.The optimal conditions were determined as follows:protease addition amount of 7800 U/g,enzymatic hydrolysis temperature of 60℃,and solid-liquid ratio of 1∶30(g/mL).Under these conditions,the ADH activation rate of the cherry stone hydrolysate reached 28.75％,which was close to the predicted value.This study provides a theoretical basis for extracting bioactive peptides from discarded cherry stone.
张媛媛;王媛;张明慧;刘嘉坤
沧州医学高等专科学校药学系,沧州 061000沧州医学高等专科学校药学系,沧州 061000沧州医学高等专科学校药学系,沧州 061000沧州医学高等专科学校药学系,沧州 061000
轻工纺织
樱桃核响应面酶解工艺醒酒多肽
cherry stoneresponse surface methodologyenzymatic hydrolysisanti-hangover peptide
《中国食品添加剂》 2026 (8)
37-43,7
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