余甘子多酚口服液工艺优化与衰老相关靶点的网络药理学分析OA

The extraction process of Phyllanthus emblica polyphenol oral liquid was optimized,the mechanism of anti-aging intervention by polyphenols from Phyllanthus emblic was analyzed using network pharmacology and molecular docking techniques.The effects of solid-liquid ratio,temperature and time on the content of total phenol in the extract of Phyllanthus emblica were investigated by single factor experiments.The factors of solid-liquid ratio,temperature and time were optimized using the Box-Bebnken center group and the experimental design method,with the total phenol content in the extract as the response value.The polyphenolic compounds and structural formulas of Phyllanthus embrica were obtained through the System Pharmacology Database and Analysis Platform of Traditional Chinese Medicine(TCMSP)and the PubChem database.The SwissTargetPrediction database was used to obtain the target sites of polyphenolic compounds from Phyllanthus emblica.Aging-related targets were searched using databases such as GeneCards.The Cytoscape_v3.9.1,String11.5 and David databases were used to construct the drug-component-gene target network diagram,protein-protein interaction(PPI)network and core targets,as well as the common targets of residual glycolic polyphenols and aging,and gene ontology(GO)analysis and KEGG enrichment analysis were conducted;the graph was visualized using the wechat platform.Molecular docking was performed using AutoDock and Pymol software.The results showed that:the optimal extraction process parameters were as follows:solid-liquid ratio of 1∶22,temperature of 45℃,and time of 53 min.Based on the above parameters,the polyphenol content in the extract of Phyllanthus emblica was measured to be 10.28±0.159 mg/mL.The research ultimately screened out 8 polyphenol compounds and 96 intersection targets of"polyphenol-aging".GO analysis revealed that the"polyphenol-senescence"intersection target was mainly involved in biological processes such as signal transduction and negative regulation of apoptosis.KEGG enrichment analysis revealed that Phyllostachya emblica mainly affected cancer pathways,endocrine resistance and other pathways.The results of molecular docking showed that the polyphenols of Phyllanthus emicus had strong binding activity with the key targets.Among them,ellagic acid had the strongest binding ability with SRC.In conclusion,the active components of Phyllanthus emblica polyphenols could interfere with aging through multi-target and multi-pathway.Among them,key active ingredients such as quercetin,subfoliar liptin,and luteolin might target genes/proteins like AKT1,SRC,and EGFR to intervene in aging.