无细胞系统催化己二酸合成1,6-己二胺研究OA

1,6-Hexanediamine is an important monomer for polyamide materials such as nylon 66,and the development of a biosyn-thetic route that is independently controllable,mild,and sustainable is of great significance.In this study,a cell-free catalytic system composed of carboxylic acid reductase and transaminase was constructed to improve the efficiency of 1,6-hexanediamine production from adipic acid.The efficiency of cascade flux transfer was enhanced through screening of key enzyme combinations and process intensifica-tion.The results showed that the titer of 1,6-hexanediamine reached 36.5 mg/L in the purified enzyme catalytic system.After the introduc-tion of the cell-free catalytic system,the kinetic matching of the cascade reaction was improved and intermediate accumulation was allevia-ted,resulting in an increase in the titer of 1,6-hexanediamine to 329.7 mg/L.After further optimization of the reaction process,the maxi-mum titer of 1,6-hexanediamine reached 571.8 mg/L at 9 h.These results lay a foundation for the green biomanufacturing of 1,6-hexane-diamine and the process intensification of cell-free systems,and provide technical support for the further development of related biomanu-facturing processes.