猴头菇多糖的制备、结构表征及其对皮质酮诱导PC12细胞损伤的保护作用OA

This study aimed to clarify the structural characteristics of Hericium erinaceus polysaccharides and their protec-tive effects against corticosterone-induced injury in PC12 cells.HEP was extracted and purified from the fruiting bodies of Hericium erinaceus by water extraction-alcohol precipitation and column chromatography.Its component contents were determined using the phenol-sulfuric acid method,Coomassie brilliant blue method,and other techniques.Structural chara-cterization was performed via Fourier transform infrared spectroscopy,ultraviolet full-wavelength scanning,molecular weight determination,scanning electron microscopy,and Congo red assay.A PC12 cell injury model induced by 200 μg/mL corticosterone was established,and the protective effects of HEP were investigated using the CCK-8 assay,lactate dehydro-genase release detection,and flow cytometry.The results showed that HEP had a total sugar content of 75.63％,containing 8.35％protein and 7.93％uronic acid,with a weight-average molecular weight of 1.94×104 Da.It possessed a β-D-glucan backbone structure,exhibited a filamentous network arrangement at the microscopic level,and contained partial triple-helix conformations.Furthermore,HEP could dose-dependently increase the viability of damaged PC12 cells(up to 81.28％),sig-nificantly reduce LDH release(down to 362.3 U/L),and decrease the cell apoptosis rate(down to 3.77％).In conclusion,HEP exerts a definite protective effect against corticosterone-induced neurocyte injury and can be further studied as a potential functional food component related to antidepressant effects.