枸杞糖肽复配咀嚼片的制备及抗疲劳活性的研究OA

This study aimed to optimize the preparation of Lycium barbarum glycopeptide complex chewable tablets and investigate their potential anti-fatigue properties.Using single-factor experiments and response surface methodology,optimal manufacturing parameters were identified based on tablets'appearance,flavor,taste and texture as a function of L.barbarum glycopeptide.The male KM mice were randomly divided into a blank group,model group,ginsenoside group(10 mg·kg-1),excipient group(400 mg·kg-1),low-dose Lycium barbarum glycopeptide compound chewable tablet group,and high-dose Lycium barbarum glycopeptide compound chewable tablet group(400,1200 mg·kg-1).A fatigue model was established by restraint combined with forced swimming for 35 consecutive days,during which the samples were adminis-tered intragastrically.After 35 days of continuous administration,a forced swimming test was conducted,and the serum levels of urea nitrogen(BUN),5-hydroxytryptamine(5-HT),lactate dehydrogenase(LDH),and creatine kinase(CK)as well as superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and malondialdehyde(MDA)content in gastrocne-mius muscle and brain tissues were determined.Results showed that,the optimal tablet formulation was constituted of 29%L.barbarum glycopeptide,17%raspberry fruit powder(sweetener),1%sea buckthorn fruit powder(acidulant),and 1.5%magnesium stearate(lubricant).Under these conditions,the comprehensive score of the chewable tablets was 85.87±0.76 points,the average tablet weight was 0.345±0.006 g,and the weight variation limit was within5%;The average hard-ness was 79.364±2.83 N,all above 75 N,with moderate hardness;The friability of the chewable tablets was less than 1%.Mouse experimental results indicated that,compared with the model group,high formulation dose significantly reduced the immobility time during forced swimming(P<0.01),decreased serum BUN,LDH and CK and tissue MDA levels(P<0.01),increased serum 5-HT level,and boosted SOD and GSH-Px activities in both muscle and brain tissues(P<0.01).L.bar-barum glycopeptide complex chewable tablets alleviated physical fatigue induced by strenuous exercise through reduction of oxidative stress in muscle and brain tissues,modulation of neurotransmitters,and improvement of energy metabolism.