钙离子诱导的裂褶菌碱溶性多糖-海藻酸钠复合水凝胶凝胶特性及其抗氧化性OA

To address the issue of the poor mechanical properties and limited functionality of sodium alginate(Alg)gels,a composite hydrogel SCAP/Alg/Ca2+that uses Alg and Schizophyllum commune alkali-soluble polysaccharide(SCAP)as substrates was prepared by induction of Ca2+.The gelation mechanism and the effects of SCAP addition amounts on the characteristics and antioxidant activity of the composite hydrogel were investigated.The results showed that the SCAP/Alg/Ca2+composite hydrogel could be formed by the interactions of ionic bonds,hydrogen bonds and hydrophobic force.The composite hydrogel exhibited a highly ordered porous network structure with the addition of SCAP and Ca2+.When the SCAP addition amount was 3%,the water holding capacity of the composite hydrogel achieved 87.32%.At pH7.4,its swelling ratios were 45.56%(wet)and 2479.82%(freeze-dried),respectively.Rheological analysis revealed a significant enhancement in the storage modulus,increasing from 455.33 Pa for pure Alg hydrogel to 17768.56 Pa for the composite hydrogel,indicating improved rheological properties.The incorporation of SCAP and Ca2+did not affect the thermal stability of the composite hydrogel but effectively enhanced its antioxidant capacity.The scavenging rate of DPPH free radicals increased from 6.95%for pure Alg to 26.65%,while the scavenging rates of ABTS+and hydroxyl free radicals also improved by approximately 10%.Furthermore,the SCAP/Alg/Ca2+composite hydrogel may also exhibit pH-respon-siveness,maintaining structural integrity in gastric fluid while undergoing rapid degradation in intestinal fluid.The findings of this study provide a scientific basis and theoretical foundation for the biomedical carrier application of SCAP/Alg/Ca2+composite hydrogels.