酵母浸粉在温度敏感型谷氨酸棒杆菌种子培养中的应用研究OA

In industrial glutamic acid production,Corynebacterium glutamicum temperature-sensitive strains serve as the primary mi-crobial hosts.Conventional nitrogen sources like soybean meal hydrolysate or corn steep liquor have been used during seed culture.Howev-er,inconsistent seed quality and frequent contamination often reduced production stability.This study evaluated the feasibility of replacing these nitrogen sources with yeast extract in the seed culture of temperature-sensitive C.glutamicum.Through spent medium analysis,the requirements for ammonium,peptides of varying molecular weights,and specific amino acids during bacterial growth were assessed.Differ-ent nitrogen sources were compared for their effects on growth rates.Key limiting nutrients were identified,leading to the selection of a suitable yeast extract.Results showed that serine,cystine,histidine,and isoleucine acted as limiting amino acids.Yeast extract FM308,rich in these amino acids,supported effective seed culture at an optimal concentration of 2 g/L.This approach shows significant potential for improving seed quality and reducing contamination in industrial glutamic acid fermentation.