胆盐胁迫下副溶血性弧菌生物被膜形成的异质性OA

To accurately investigate the heterogeneity of biofilm formation by Vibrio parahaemolyticus under various concentrations of bile salt stress,this study selected six strains isolated from seawater,freshwater,and clinical samples.Crystal violet staining and the Cell Counting Kit-8(CCK-8)assay were employed to determine the differences in biofilm-forming capacity and cell viability under varying bile salt concentrations.Additionally,confocal laser scanning microscopy(CLSM)was utilized to observe the biofilm architecture and analyze changes in biofilm biomass and structural parameters under bile salt stress.The results indicated that bile salts inhibited biofilm formation in both environmental and clinical V.parahaemolyticus strains,leading to a significant reduction in biofilm biomass and cell viability,while delaying the time required to reach peak biofilm mass.Under high-concentration bile salt stress,clinical strains exhibited greater bio-film-forming capacity than environmental strains;however,under low-concentration bile salt stress,environmental strains outperformed clinical strains in biofilm formation.Furthermore,strains containing the tdh gene demonstrated a rapid response to and stronger tolerance against bile salt stress.These findings reveal the heterogeneity in the biofilm formation process and the time to reach maximum biofilm bio-mass among V.parahaemolyticus strains under varying bile salt stress levels,providing novel insights for the safety control of V.parahaemo-lyticus and targeted interventions for host intestinal infections.