植物乳植杆菌F44的抑菌特性及其细菌素基因簇的挖掘OA

This study aimed to screen lactic acid bacteria that have an inhibitory effect on the dominant spoilage bacterium of chilled chicken,Pseudomonas lundensis,analyze their antibacterial characteristics,and mine potential bacteriocin gene clusters based on whole-genome sequences,ultimately evaluating their effectiveness in preserving chilled chicken.The results indicated that the selected Lactipla-ntibacillus plantarum F44 had a significant inhibitory effect on P.lundensis,with a minimum inhibitory concentration value of 312 μg/mL.The crude bacteriocin extract was sensitive to proteases,remained highly active against bacteria at 40-121 ℃ and pH range of 2.0-10.0,and exhibited antibacterial activity against common Gram-positive and Gram-negative bacteria.Whole-genome analysis showed that strain L.plantarum F44 had a genome length of 3 746 814 bp,a GC content of 41.33％,and contained five bacteriocin gene clusters.Af-ter treatment of chilled chicken with F44 bacteriocin(2MIC),the total bacterial count decreased by 2.6 lg CFU/g and the thiobarbituric acid reactive substances content decreased by 0.35 mg/kg after 12 days of refrigeration,extending the shelf life by 3 days.In summary,L.plantarum F44 and the bacteriocins it produces have good antibacterial properties and preservation potential,providing new microbial resources and a theoretical basis for the development of natural preservatives,and have promising application prospects.