玉米须发酵液对2型糖尿病小鼠糖脂代谢的调节作用OA

To explore the hypoglycemic effect of corn silk fermentation liquid(CSFL)on diabetic mice and its underlying mechanism,a type 2 diabetic mouse model was induced by a high-fat diet combined with streptozotocin.The effects of different doses of CSFL on glucose and lipid metabolism,liver function,histopathology,and intestinal microbiota composition were examined.The results showed that CSFL reduced food and water intake of diabetic mice to varying degrees.Compared with the model group,the high-dose CSFL group exhibited significant reductions in fasting blood glucose(FBG),total cholesterol,and low-density lipoprotein cholesterol(P<0.001),along with a significant improvement in glucose tolerance(P<0.001).The activity of catalase was markedly elevated(P<0.05).Both high-and low-dose CSFL groups showed significant increases in glutathione peroxidase activity(P<0.001),significant reduction in malondialdehyde content(P<0.01,P<0.001),and significant decreases in aspartate aminotransferase activity(P<0.05).In both CSFL groups,the liver index was significantly increased(P<0.001),while no significant differences were observed in the pancreas,kidney,or spleen indices(P>0.05).CSFL administration effectively alleviated pathological changes such as hepatic steatosis and pancreatic islet cell damage.Compared with the model group,the high-dose CSFL group significantly increased the Chao1,Simpson,and Shannon indices of the gut microbiota(P<0.05,P<0.01).CSFL administration decreased the relative abundance of Bacteroidota and increased the relative abundance of Verrucomicrobiota and Akkermansia in the gut of diabetic mice.The contents of acetic acid,propionic acid,butyric acid,and isobutyric acid in mouse feces were significantly increased by CSFL(P<0.05,P<0.001).In conclusion,CSFL can regulate glucose and lipid metabolism,strengthen antioxidant capacity and remodel intestinal microbiota in a dose-dependent manner,indicating its potential for development as a natural hypoglycemic product.