内蒙古驼奶源高血管紧张素转换酶抑制活性益生菌筛选及其发酵条件优化OA

To screen probiotic strains with high angiotensin-converting enzyme(ACE)inhibitory activity,539 preserved probiotic strains in laboratory were used as research materials.Strains with strong ACE inhibitory activity were preliminarily screened according to ACE inhibition rate and further identified via physiological-biochemical tests and molecular biological methods.The fermentation conditions for the selected strain were optimized using single-factor experiments,Plackett-Burman design,steepest ascent experiment,and Box-Behnken design.The results showed that strain Y43-3-1,which exhibited the highest ACE inhibitory activity,was isolated and identified as Lactiplantibacillus plantarum.The optimal fermentation conditions were as follows:glucose 1.9 g/100 mL,yeast powder 12 g/L,fermentation duration 28 h,incubation temperature 37℃,inoculation volume 2%(V/V),initial pH 6.0.Under optimized conditions,the ACE inhibition rate reached 65.72%,which was significantly higher than the initial value of 58.16%.In summary,L.plantarum Y43-3-1 with potent ACE inhibitory activity was screened out,and its fermentation conditions were optimized in this study.This work provided excellent strain resources and theoretical support for its development and application in antihypertensive functional foods or probiotic preparations.