榆耳多糖对DSS诱导小鼠溃疡性结肠炎的改善作用OA

To investigate the ameliorative effects of Gloeostereum incarnatum polysaccharides(GIP)on ulcerative colitis induced by 3％dextran sulfate sodium salt(DSS)in mice,animals were randomly assigned into the following groups:blank control(CON),DSS model(DSS),low-dose GIP(GIPL,50 mg·kg-1),medium-dose GIP(GIPM,100 mg·kg-1),high-dose GIP(GIPH,200 mg·kg-1),and a positive control group treated with salazosulfapyridine(SASP,200 mg·kg-1).Enzyme-linked immunosorbent assay(ELISA)was used to determine serum levels of pro-inflammatory cytokines(TNF-α,IL-6),content of the oxidative stress marker malondialdehyde(MDA),and the activities of diamine oxidase(DAO),lactate dehydrogenase(LDH),reduced glutathione(GSH),and superoxide dismutase(SOD).Western blot analysis was conducted to detect the expression of proteins related to the NF-κB signaling pathway and the intestinal tight junction-related proteins ZO-1 and Occludin.The results showed that compared with the DSS model group,GIPM and GIPH significantly ameliorated colonic tissue damage,with body weight notably restored in the GIPH group.Both GIPM and GIPH groups exhibited significantly reduced disease activity index(DAI)scores and DAO activity.Histopathological examination revealed that GIPM and GIPH treatment repaired colonic tissue architecture,ameliorated mucosal necrosis and inflammatory cell infiltration,and restored normal tissue morphology.GIPM and GIPH also significantly increased GSH activity,and all GIP dose groups(GIPL,GIPM,GIPH)significantly increased SOD activity while decreasing MDA content and LDH activity.Serum levels of the pro-inflammatory cytokines IL-6 and TNF-α were also significantly decreased across all GIP groups.GIPM and GIPH upregulated the expression of the intestinal tight junction proteins ZO-1 and Occludin,and suppressed the activation of the NF-κB inflammatory signaling pathway.These findings suggest that GIP effectively alleviates DSS-induced UC by modulating oxidative stress,suppressing inflammation,and restoring intestinal barrier integrity through the NF-κB signaling pathway,providing a reference for the development of G.incarnatum as a functional food or therapeutic agent for ulcerative colitis.