三种蜜环菌子实体对氯苯丙氨酸诱导失眠小鼠的影响OA

The effects of three Armillaria species—Armillaria gallica,A.ostoyae,and A.desertorum—on para-chlorophenylalanine(PCPA)-induced insomnia were studied in a mouse model.Mice were intragastrically administered fruiting body powders of the three species at high(2.0 g·kg-1)and low(1.0 g·kg-1)doses.Body mass and Morris water maze escape latency were measured.Serum levels of interleukin-6(IL-6),interleukin-1β(IL-1β),and tumor necrosis factor-α(TNF-α),as well as hippocampal levels of 5-hydroxytryptamine(5-HT),γ-aminobutyric acid(GABA),norepinephrine(NE),dopamine(DA),and glutamic acid(Glu),were determined.Histopathological changes in the liver,heart,and hippocampus were observed using hematoxylin-eosin(HE)staining.The results showed that,compared with the model group,all treatment groups showed significant increases in body mass and significant reductions in escape latency.All three Armillaria species decreased the levels of IL-6,IL-1β and TNF-αin serum.They elevated hippocampal levels of 5-HT and GABA while decreasing NE,DA and Glu levels.They also ameliorated insomnia-induced damage to the liver,heart and hippocampus.These findings provide a scientific reference for the further development and utilization of Armillaria species.