温度和外源γ-氨基丁酸对黄伞菌丝生长和胞外酶活力的影响OA

Using a two-factor analysis of variance,the effects of temperature and exogenous γ-aminobutyric acid(GABA)on mycelial biomass accumulation,GABA content,peptide content in fermentation broth,and extracellular enzyme activity of Pholiota adiposa mycelia under submerged liquid culture were studied.The results showed that supplementation with 20 mmol·L-1 exogenous GABA followed by incubation at 28 ℃ for 12 d yielded the highest mycelial biomass(7.84 mg·mL-1),GABA content(0.53 mg·mL-1),and peptide content(4.92 mg·mL-1)in the fermentation broth.Under the same exogenous GABA supplementation concentration and incubation temperature,maximal amylase and xylanase activities were observed at 6 d(3 433.31 and 19 197.91 U·L-1 respectively),while peak cellulase activity was observed at 9 d(3 203.03 U·L-1).In contrast,supplementation with 60 mmol·L-1 exogenous GABA at 35 ℃ inhibited mycelial growth and reduced extracellular enzyme activities.These findings provide a reference for optimizing the submerged liquid culture process of P.adiposa and may facilitate the development of efficient cultivation protocols for this commercially valuable mushroom species.