集约化培养香菇菌棒的理化指标及其相关性OA

To investigate the respiration intensity,physicochemical indicators,and their correlation in Lentinula edodes cultivation sticks(inoculated bags filled with culture substrate)under intensive cultivation,the following parameters of the cultivation sticks were measured throughout the cultivation process:respiration intensity,moisture content,pH,glucose content,D-xylose content,organic carbon content,total nitrogen content,cellulose content,hemicellulose content,lignin content,ethanol content,lactic acid content,ATP content,carboxymethyl cellulase activity,xylanase activity,laccase activity,and Na+/K+-ATPase activity.The correlations among these indicators were also analyzed.The results showed that respiration intensity initially increased and then decreased,reaching its maximum at 43 d of cultivation(2 d after the first aeration treatment by punching holes on the cultivation bags).By 88 d of cultivation,moisture content,pH,C/N ratio,and the contents of cellulose,hemicellulose,and lignin were all lower than those at 10 d.The contents of D-xylose and ethanol peaked at 40 d of cultivation,while the contents of glucose and lactic acid,along with the activities of carboxymethyl cellulase,xylanase,and laccase,reached their highest levels at 88 d of cultivation.ATP content and Na+/K+-ATPase activity peaked at 42 d of cultivation.Respiration intensity showed a significant positive correlation with Na+/K+-ATPase activity and ATP content(r≥0.79,P＜0.05),and a significant negative correlated with pH(r=-0.72,P＜0.05).These findings provide a reference for the intensive cultivation of L.edodes.