高产三萜灵芝新菌株'沪农芝8号'选育OA

To develop a novel Ganoderma lucidum strain with improved contamination resistance,high triterpenoid content,and superior biological efficiency,protoplast monokaryon hybridization was performed using'Hunong Lingzhi No.1'(H1)and G0130 as parent strains.H1 is characterized by strong contaminant resistance and high biological efficiency,whereas G0130 has high triterpenoid content but weak contaminant resistance and relatively low biological efficiency.From 124 hybrid combinations,strains with superior mycelial growth were initially selected and subsequently subjected to cluster analysis using inter-simple sequence repeat(ISSR)molecular markers.Based on the biological characteristics of the mycelia,82 representative hybrid strains were selected across all cluster groups for cultivation trials.After systematic evaluation of biological efficiency,fruiting body yield,and triterpenoid content,a hybrid strain named GH20 was ultimately identified as the candidate with outstanding comprehensive traits.The triterpenoid content of GH20 fruiting bodies reached 1.68％-1.80％,approximately a 100％increase over H1,while remaining comparable to that of G0130.GH20 also showed excellent contaminant resistance,characterized by white,robust,and luxuriant mycelia with high growth rates,high fruiting uniformity,and a biological efficiency of up to 9.00％,equivalent to that of H1.Multi-site demonstration trials confirmed the stability and consistency of these superior traits.In 2020,GH20 was certified by the Shanghai Municipal Non-Major Crop Variety Approval Committee and officially designated as'Hunongzhi No.8'.