黑木耳'云耳1号'和'云耳2号'选育OA

Wild fruiting bodies of Auricularia were collected from Funing County,Wenshan Zhuang and Miao Autonomous Prefecture,and Jinjiang Town,Shangri-La City,Diqing Prefecture,Yunnan Province,China.Pure strains L7061,L7013,L7062 and L7120 were obtained through tissue isolation and identified as A.heimuer.Using the commercial cultivar'Heibao 1'as the control,the candidate strains were evaluated through a sequential process consisting of primary screening,secondary screening,differential identification,pilot-scale cultivation,and demonstration cultivation,followed by a comprehensive nutritional analysis.The results showed that strain L7061 produced solitary,single-piece fruiting bodies characterized by minimal back-side wrinkles,a small stipe base,entire margins,and a deep brown color.The fruiting body diameter was(7.26±0.5)cm,thickness was(0.16±0.02)cm,and the biological efficiency reached(114.3±1.29)％.Strain L7013,by contrast,produced clustered fruiting bodies with wrinkled backs,thick and black pilei,a diameter of(6.2±0.5)cm,a thickness of(0.18±0.04)cm,and a biological efficiency of(123.5±1.41)％.In terms of nutritional composition,L7061 contained 17.60 g·100 g-1 protein and 48 g·100 g-1 crude polysaccharides,while L7013 contained 20.50 g·100 g-1 protein and 45.6 g·100 g-1 crude polysaccharides,both significantly exceeding the values recorded for the control variety.On April 12,2024,strains L7061 and L7013 passed the non-major crop variety certification of Yunnan Province and were officially named'Yun'er 1'and'Yun'er 2',with certification numbers Dianjian(Edible Fungi)2024042 and 2024043,respectively.