香菇孢子单核体菌株菌丝生长速度配合力分析OA

Using a North Carolina Ⅱ(NC Ⅱ)experimental design,eight spore-derived monokaryotic strains isolated from the Lentinula edodes cultivar'Shenxiang 215'were crossed with eight compatible spore-derived monokaryotic strains through an incomplete diallel cross,yielding 64 hybrid combinations.Mycelial growth rates of the hybrid progeny were measured on both potato dextrose agar(PDA)plates and sawdust-based medium.General combining ability(GCA)of the spore-derived monokaryotic strains,specific combining ability(SCA)of the hybrid combinations,and genetic variance components of mycelial growth rate were analyzed.The results showed extremely significant differences in mycelial growth rate between the tested strains.There was no significant correlation between the mycelial growth rates of the spore-derived monokaryotic strains and that of their hybrid progeny on PDA plates.However,mycelial growth rates of the hybrids on PDA plates and sawdust medium were extremely significantly correlated.Both the GCA between the tested spore-derived monokaryotic strains and the SCA between the hybrid combinations were extremely significantly different.The genetic effects governing mycelial growth rate on PDA plates were predominantly additive,with a narrow-sense heritability of 63.18％.In contrast,mycelial growth on sawdust medium was affected by both genetic background and culture environment,with a narrow-sense heritability of 38.97％.There was no correlation between the GCA of the spore-derived monokaryotic strains and the SCA of their corresponding hybrid combinations.Four spore-derived monokaryotic strains,M4-4,M2-2,Csm82,and Csm89,showed relatively high GCA values,suggesting their potential as elite parent strains for breeding new germplasm with improved mycelial growth.