高效液相色谱法同时测定西河柳叶中5种黄酮类成分含量OA

In order to establish a high-performance liquid chromatography(HPLC)analytical method for the simultaneous determination of quercetin,luteolin,kaempferol,tamarixin and kaempferide contents in Tamaricis cacumen leaves,the quercetin,luteolin,kaempferol,tamarixin and kaempferide contents in Tamaricis cacumen leaves were determined on a Shim-pack GIST C18(4.6 mm×150 mm,5 μm)with methanol-0.01%K2HPO4 solution(gradient elution)as the mobile phase at a flow rate of 0.5 mL/min,a detection wavelength of 366 nm,and a column temperature of 35℃with a 10 μL injection volume.The results showed that the quercetin,luteolin,kaempferol,tamarixin and kaempferide showed good linear relationship with peak area in the range of 0.0017～0.1632 mg/mL(R2≥0.9987);the RSD values of their precision were no more than 1.8%;the RSD values of their reproducibility were no more than 1.8%;the spiked recoveries of quercetin,luteolin,kaempferol,tamarixin and kaempferide were in the range of 95%～105%with RSD no more than 2.0%.The samples had good stability when placed at room temperature for 12 h.In conclusion,this study established a method for the simultaneous determination of five flavonoids in Tamaricis cacumen leaves.The method demonstrated simplicity,high sensitivity,accuracy,strong specificity,and good repeatability,complying with the relevant standards of the 2020 Pharmacopoeia and satisfying analytical requirements.