畜禽骨蛋白水解在多肽螯合钙粉剂制备中的应用研究OA

This study investigated the application effects of livestock and poultry bone protein hydrolysis in the preparation of polypeptide-chelated calcium powder,livestock and poultry bone such as cattle bone and deer bone were used as raw materials,a two-stage enzymatic hydrolysis process was employed to hydrolyze the bone proteins,extract bone collagen,and prepare peptides.Supported by chelation mechanisms,the peptide enzymatic hydrolysate was mixed with calcium chloride solution and subsequently dried to obtain the polypeptide-chelated calcium powder.The performance of the resulting polypeptide-chelated calcium powder was evaluated against a control group(prepared without bone protein hydrolysis)across four dimensions:calcium chelation rate,absorption rate,stability and safety.Comprehensive analysis of polypeptide-chelated calcium powders derived from cattle bone,deer bone,cod bone,and tuna bone showed that the application of bone protein hydrolysis yielded powders with an average calcium chelation rate of 61.35%,an average particle size of approximately 35.74 nm,an average thermal stability coefficient of 0.89,and an average heavy metal content of about 0.088 mg.In contrast,the control group exhibited a calcium chelation rate of 34.5%,an average particle size of 67.34 nm,a thermal stability coefficient of 0.79,and a heavy metal content of 0.18 mg.Therefore,the application of livestock and poultry bone protein hydrolysis significantly enhanced the calcium chelation rate of polypeptide-chelated calcium powder,while improving its absorption characteristics,stability and safety profile.These findings demonstrated the favorable application potential of bone protein hydrolysis in the preparation of polypeptide-chelated calcium powders.