杜仲叶提取物自组装纳米载体的筛选、鉴定及其对姜黄素的包埋应用OA

A small-molecule self-assembled nanomaterial(SMSN)-enriched extract from Eucommia ulmoides Oliv.leaves(EuNE)was isolated and purified as an alternative to high-purity SMSNs,with the aim of reducing production costs and facilitating industrial application.A self-assembly performance-guided fractionation strategy was applied to screen and identify functional EuNE components,which were further characterized using chromatographic techniques.The encapsulation efficiency and stability of curcumin loaded into EuNE self-assembled nanoparticles(EuNE NPs)were systematically evaluated.The EuNE fraction obtained via methanol extraction,petroleum ether fractionation,and ethanol precipitation exhibited excellent self-assembly capability.The resulting EuNE NPs showed an average particle size of 232.79 nm and a surface charge of--30.13 mV.Oleanolic acid(38.56 mg·g-1)and ursolic acid(68.35 mg·g-1)were identified as the major carrier components,forming nanoparticles through synergistic hydrogen-bonding and electrostatic interactions.Curcumin encapsulation efficiency in EuNE NPs reached 77.40％,accompanied by markedly improved stability under ultraviolet irradiation,elevated temperatures,different pH conditions,and varying ionic strengths.In simulated gastric fluid,69.34％of curcumin was released within 3 h,while cumulative release in simulated intestinal fluid reached 44.60％over the same period,demonstrating sustained-release behavior.These results indicate that SMSN self-assembly can be achieved without high-purity monomeric components and highlight the potential of Eucommia leaf extracts for the development of cost-effective,high-performance natural nanocarrier systems.