萨能山羊乳中低聚糖的靶向绝对定量研究OA

To investigate the specific oligosaccharide composition,content,and proportional distribution in Saanen goat milk and to perform a comparative analysis with Holstein bovine milk,targeted absolute quantification of 23 target oligosaccharides was performed in Saanen goat milk and Holstein bovine milk using ultra-performance liquid chromatography-hydrophilic interaction chromatography-triple quadrupole mass spectrometry(UPLC-HILIC-QqQ MS).The method exhibited a limit of quantification as low as 10.0 ng/mL,recoveries ranging from 83.98％to 111.44％,and satisfactory precision,meeting the requirements for accurate quantitative detection of milk oligosaccharides.Eight oligosaccharides were detected in both Saanen goat milk and Holstein bovine milk,including 2'-fucosy llactose(2'-FL),3'-fucosyllactose(3'-FL),lacto-N-neotetraose(LNnT),6'-galactosyllactose(6'-GL),4'-galactosyllactose(4'-GL),lacto-N-hexaose(LNH),3'-sialyllactose(3'-SL),and 6'-sialyllactose(6'-SL).The total content of these eight oligosaccharides in Saanen goat milk was(109.28±10.00)mg/L,which was significantly higher than that in Holstein bovine milk(44.87±2.87)mg/L,2.44 times that of the latter value(P＜0.05).The relative proportions of neutral and acidic oligosaccharides were(42.96±2.50)％and(57.04±1.57)％in Saanen goat milk,and(32.44±4.37)％and(67.56±5.68)％in Holstein bovine milk,respectively.Furthermore,the mass concentrations of 2'-FL,4'-GL,6'-GL,6'-SL and 3'-SL in Saanen goat milk were 2.03,6.20,2.53,2.05 and 2.07 times those in Holstein bovine milk,respectively(P＜0.05).The findings demonstrated that compared with Holstein bovine milk,Saanen goat milk not only had a higher total oligosaccharide content,but also a higher proportion of neutral oligosaccharides,highlighting its significant potential as a high-quality dairy source for infant formula and other functional food products.