灵芝多糖对不同类型益生菌生长及代谢的影响OA

Using inulin as the basal carbon source,the optimal addition amount was screened for different species of probiotics.Based on the optimal inulin addition amount for each probiotic species,Ganoderma lucidum polysaccharide(GLP)was co-supplemented,and its effects were compared with a high inulin addition group,where the inulin addition amount equaled the sum of the optimal inulin addition amount from the screening experiments and the addition amount of GLP.By measuring bacterial biomass(D600),total sugar consumption,and contents of short-chain fatty acids(SCFAs),the effects of GLP on the growth and metabolism of seven probiotic strains(Lactobacillus gasseri,Bifidobacterium adolescentis,Clostridium butyricum,Bacteroides fragilis,B.ovatus,B.thetaiotaomicron and B.uniformis)were investigated.The results showed that GLP was consumed and utilized by all seven probiotic species.It significantly promoted the growth of L.gasseri,C.butyricum,B.fragilis,B.ovatus and B.uniformis.The total SCFAs contents in the GLP groups for all seven probiotic species were significantly higher than those in the optimal inulin addition groups.Furthermore,the total SCFAs contents in the GLP groups of B.fragilis and B.uniformis were also significantly higher than those in their high inulin groups(increased by 7.67 and 4.59 mmol·L-1,respectively,corresponding to relative increases of 58.61％and 19.89％).These findings provided a reference for the development of GLP as a targeted prebiotic preparation.