大白桩菇多酚提取工艺优化及抗氧化和抑制癌细胞增殖活性OA
Optimization of Polyphenol Extraction from Leucopaxillus giganteus and Evaluation of Antioxidant and Antiproliferative Activities
在单因素实验基础上,选择乙醇体积分数、料液比、超声时间3个因素,采用响应面法优化大白桩菇(Leucopaxillus giganteus)多酚提取工艺,并测定大白桩菇多酚提取液对DPPH自由基清除率和铁离子还原能力(FRAP)以及对体外培养膀胱癌T24细胞的抑制率.结果表明:多酚最优提取工艺为乙醇体积分数60％、料液比1∶15(g·mL-1)、超声时间30 min,该条件下制备的提取液多酚含量达到6.383 μg·mL-1;在实验范围内,50 mg·mL-1多酚提取液对DPPH自由基的清除率最高,为94.21％;对铁离子还原能力最大,为2.14 mmol·100 g-1;多酚提取液对膀胱癌T24细胞抑制的IC50为0.274 mg·mL-1.研究结果可为大白桩菇的进一步开发提供参考.
Based on single-factor experiments,three factors-ethanol volume fraction,solid-liquid ratio,and ultrasonic time-were selected to optimize the polyphenol extraction process from Leucopaxillus giganteus using response surface methodology.The antioxidant activity of the obtained polyphenol extract was evaluated by determining its DPPH radical scavenging rate,ferric reducing antioxidant power(FRAP),and its inhibitory rate on the proliferation of T24 human bladder cancer cells in vitro.The results showed that the optimal extraction conditions were as follows:60％ethanol volume fraction,solid-liquid ratio of 1∶15 g·mL-1,and ultrasonic time 30 min.Under these conditions,the polyphenol content reached 6.383 μg·mL-1.Within the experimental range,the polyphenol extract showed the highest DPPH free radical scavenging rate of 94.21％and the maximum FRAP value of 2.14 mmol·100 g-1 at 50 mg·mL-1.The IC50 of the polyphenol extract against T24 cells was 0.274 mg·mL-1.These findings provided a reference for further development of Leucopaxillus giganteus.
张雅君;古丽拜尔·艾赛提;李艳婷;郭尚
山西农业大学山西功能食品研究院,山西太原 030031新疆大学生态与环境学院,新疆乌鲁木齐 830017山西农业大学山西功能食品研究院,山西太原 030031山西农业大学山西功能食品研究院,山西太原 030031
大白桩菇多酚响应面优化抗氧化活性膀胱癌T24细胞
Leucopaxillus giganteuspolyphenolsresponse surface optimizationantioxidant activityT24 human bladder cancer cell line
《食用菌学报》 2026 (3)
66-74,9
山西省基础研究计划青年科学研究项目(202403021222095、202103021223139)
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