红托竹荪菌托多糖提取、结构表征及体外生物活性OA

Polysaccharide from volva of Dictyophora rubrovolvata(DRVP)was extracted and purified by water extraction and alcohol precipitation,and the structural characteristics and biological activities of DRVP were investigated.Structural characterization revealed that DRVP had a dense,irregular flake-like structure with a smooth surface.It was primarily composed of monosaccharides including glucose,glucuronic acid,galactose,and mannose.DRVP exhibited heterogeneous molecular weight distribution with significant polydispersity.Fourier transform infrared spectroscopy analysis indicated that DRVP was a typical polysaccharide with pyranose ring skeleton,containing both α-and β-glycosidic linkages.In in vitro bioactivity assays,DRVP showed strong antioxidant activity,with IC50 values for scavenging ABTS and DPPH radicals being 0.307 mg·mL-1 and 0.449 mg·mL-1,respectively.DRVP displayed significant inhibitory activity against α-glucosidase and α-amylase,with IC50 values of 0.0865 mg·mL-1 and 0.0832 mg·mL-1,respectively.DRVP also inhibited pancreatic lipase and effectively bound bile salts:at 20 mg·mL-1,the inhibition rate on pancreatic lipase reached(38.23±2.01)％,and the binding rates to sodium glycocholate and sodium taurocholate were(47.55±0.69)％and(51.67±0.60)％,respectively.These results indicated that DRVP possesses favorable biological activities and broad application prospects in the fields of functional foods and pharmaceuticals.