黄色品种金针菇'上研金31'选育OA
Breeding of the Yellow Variety Flammulina filiformis'Shangyan Jin31'
以高产金针菇(Flammulina filiformis)'上研1号'(白色)和菌盖厚实、菌柄粗壮的金针菇'J4137'(黄色)为亲本,经原生质体单核化后单单杂交获得杂交菌株,经初筛、复筛、中试和示范栽培选育菌株.结果表明:在工厂化生产模式下,与'上研1号'相比,选育出的菌株F31的原基形成时间和子实体生育时间均缩短2d;与'J4137'相比,菌株F31的产量和子实体数量均增加;与2个亲本相比,菌株F31的氨基酸总含量和赖氨酸含量均增加.在工厂化袋栽模式下,菌株F31每袋产量达297.8 g,子实体数量为424个,子实体生育时间为28 d;在工厂化瓶栽模式下,每瓶产量达459.8 g,子实体数量为1 025个,子实体生育时间为25d.菌株F31栽培性状稳定、适应性强,适合工厂化栽培需求.2024年,菌株F31通过上海市非主要农作物品种认定,定名为'上研金31'.
Using the high-yielding Flammulina filiformis variety'Shangyan 1'(white)and the thick-pileus and sturdy-stipe F.filiformis variety'J4137'(yellow)as the parents,hybrid strains were obtained through mon-mon hybridization of monokaryotic protoplasts.These hybrid strains were then selected for high yield,thick pileus,uniform color,and suitability for industrial cultivation through primary screening,secondary screening,pilot-scale experiment,and demonstration cultivation.The results showed that under industrial cultivation conditions,compared with'Shangyan 1',the selected strain F31 exhibited a two-day reduction in both primordium formation time and fruiting body formation period.Compared with'J4137',F31 had a higher yield and increased number of fruiting bodies.Compared with the two parents,fruiting bodies of F31 showed a higher total amino acid content and lysine content.Under industrial bag cultivation conditions,F31 achieved a yield of 297.8 g,with 424 fruiting bodies and a fruiting body formation period of 28 d.Under industrial bottle cultivation conditions,it reached a yield of 459.8 g,with 1 025 fruiting bodies and a fruiting body formation period of 25 d.These results indicate that F31 has stable cultivation characteristics,strong adaptability,and is suitable for industrial-scale cultivation.In 2024,strain F31 was approved as a non-major crop variety in Shanghai,and was officially named'Shangyan Jin31'.
陆欢;尚晓冬;刘建雨;董慧;王瑞娟;胡卫红;潘峰;冯占
上海市农业科学院食用菌研究所,囯家食用菌工程技术研究中心,上海 201403上海市农业科学院食用菌研究所,囯家食用菌工程技术研究中心,上海 201403上海市农业科学院食用菌研究所,囯家食用菌工程技术研究中心,上海 201403上海市农业科学院食用菌研究所,囯家食用菌工程技术研究中心,上海 201403上海市农业科学院食用菌研究所,囯家食用菌工程技术研究中心,上海 201403上海雪榕生物科技股份有限公司,上海 201401天水众兴菌业科技股份有限公司,甘肃天水 741000江苏华绿生物科技股份有限公司,江苏宿迁 223700
金针菇原生质体杂交农艺性状赖氨酸
Flammulina filiformisprotoplasthybridizationagronomic traitslysine
《食用菌学报》 2026 (3)
21-32,12
上海市农业科技创新项目(2025-02-08-00-12-F00022)上海市农业科学院卓越团队建设项目(沪农科卓[2022]001)
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