基于在线净化结合液相色谱-质谱技术识别茶叶中非法添加的9种合成色素OA
Identification of 9 Synthetic Pigments Illegally Added to Tea Based on Online Purification Combined with Liquid Chromatography-Mass Spectrometry Technology
建立了在线净化结合液相色谱-串联质谱法测定茶叶中非法添加的柠檬黄、日落黄等 9 种合成色素的分析方法.样品经水浸泡,氨水甲醇溶液提取,Cyclone-P 柱在线净化后,采用液相色谱-串联质谱法测定.在优化后的试验条件下,9 种色素在 0.010～1.0 μg·mL-1 呈现出良好的线性关系,其相关系数均大于 0.999;9 种色素的定量限(LOQ)为 0.10～0.50 mg·kg-1,平均回收率在 71.5%～105.9%,相对标准偏差≤8.7%.该方法简便、快速,选择性和重现性好,灵敏度高,结果准确可靠,对于茶叶样品中非法添加合成色素的定量检测具有明显优势.
A liquid chromatography-tandem mass spectrometry method for the determination of nine synthetic pigments illegally added to tea,such as tartrazine and sunset yellow by online purification was established.The samples were first soaked in water,then extracted with ammonia water and methanol solution,purified online by Cyclone-P column,and then determined by liquid chromatography-tandem mass spectrometry(LC-MS/MS).Under optimized conditions,the nine pigments showed a good linear relationship within the mass concentration range of 0.010-1.0 μg·mL-1,with correlation coefficients greater than 0.999.The limits of quantitation(LOQ)of nine colorants were 0.10-0.50 mg·kg-1.The mean recoveries ranged from 71.5%to 105.9%,with the relative standard deviation of≤8.7%.This method is simple,rapid,has good selectivity and reproducibility,high sensitivity,accurate and reliable results.It has obvious advantages for the quantitative detection of illegal added pigments to tea samples.
黄超群;吴娟;陈红平;裘睿涵;黄佳波;王依;陈丽;韩超
杭州海关技术中心,浙江 杭州 310016杭州海关技术中心,浙江 杭州 310016中国农业科学院茶叶研究所,浙江 杭州 310008杭州海关技术中心,浙江 杭州 310016杭州海关技术中心,浙江 杭州 310016杭州海关技术中心,浙江 杭州 310016杭州海关技术中心,浙江 杭州 310016浙江树人学院生物与环境工程学院,浙江 杭州 310015
农业科技
液相色谱-串联质谱在线净化茶叶色素
liquid chromatography-tandem mass spectrometry(LC-MS/MS)online purificationteapigment
《茶叶科学》 2026 (3)
499-508,10
海关总署科研项目(2022HK123)
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