鼠李糖乳酪杆菌B6胞外多糖对脂多糖诱导Caco-2细胞损伤的影响OA

To explore the protective effects and underlying mechanisms of Lactobacillus exopolysaccharides against lipopolysaccharide-induced intestinal barrier damage,a pure and structurally well-characterized polysaccharide(B6-EPS)isolated and purified from Lactica-seibacillus rhamnosus B6 was used as the research subject.The improvement effect of B6-EPS on LPS-induced injury in Caco-2 cell was systematically evaluated from multiple perspectives in vitro,including cell viability,inflammatory factor levels,transepithelial electrical resistance and permeability of Caco-2 cell monolayers,and tight junction protein expression.The results demonstrated that intervention with 200 μg/mL B6-EPS could significantly improve the viability of Caco-2 cells(P＜0.05),enhance the level of anti-inflammatory fac-tor IL-10(P＜0.01),and promote the expression of tight junction protein CLDN3 to improve the integrity of Caco-2 cell monolayers,thereby alleviating LPS-induced Caco-2 cell injury.In addition,the results of gene differential analysis also provided potential new targets for B6-EPS in regulating cellular inflammation and oxidative stress.Overall,the findings of this study provide scientific support for the pro-tective effect of probiotic exopolysaccharides against lipopolysaccharide-induced intestinal barrier damage,and lay a foundation for further investigations into the deep mechanisms by which CLDN3 mediates the regulation of intestinal barrier function.