'琐琐'葡萄对聚乙二醇模拟干旱胁迫的形态与生理响应OA

In order to clarify the theoretical basis of'Suosuo'grape as an excellent drought-resistant rootstock,the morphological and physiological responses of'Suosuo'grape test-tube seedlings under polyethylene glycol(PEG)simulated drought stress were investigated.The test-tube seedlings of'Suosuo'grape were used as test materials,and different mass concentrations of PEG-6000 were added to the medium to simulate drought stress.The drought injury index,cell membrane permeability,relative water content,osmotic adjustment substances,malondialdehyde and antioxidant enzyme activity were measured and comprehensively evaluated.The results showed that with the increase of PEG concentration and the prolongation of stress time,the relative conductivity,malondialdehyde,proline and soluble sugar continued to rise,the relative water content decreased significantly,the soluble protein decreased first,then increased and then decreased,and the activities of three antioxidant enzymes(SOD,POD and CAT)increased first and then decreased,and there were differences among different stress treatments.Based on grey correlation analysis,principal component analysis and correlation analysis,five indexes of relative conductivity,malondialdehyde,proline,soluble sugar and relative water content were selected to constitute the comprehensive evaluation system of drought resistance of'Suosuo'grape,among which the correlation between relative conductivity and drought injury index was the highest.In summary,'Suosuo'grape has significant cell membrane stability and osmotic adjustment ability under drought stress,and has the potential to be developed as an excellent drought-resistant rootstock.