上海地区长期避雨栽培下葡萄园土壤特征与细菌群落的阶段性演变OA

To investigate the stage-specific effects of long-term rain-shelter cultivation on soil properties and bacterial communities in vineyards in Shanghai,this study examined soils from'Jumeigui'vineyards under open-field cultivation,rain-shelter cultivation for 6 years,and rain-shelter cultivation for 12 years.Soil physicochemical properties,enzyme activities,and bacterial community structure were analyzed using conventional soil analyses and absolute quantitative 16S rRNA sequencing.The results showed that,compared with open-field cultivation,rain-shelter cultivation significantly decreased soil pH and increased electrical conductivity and soluble salt content,with salt accumulation becoming more pronounced as the duration of rain-shelter cultivation increased.Rain-shelter cultivation had differential effects on soil nutrients and enzyme activities.In the 12-year rain-shelter cultivation treatment,total nitrogen,total phosphorus,available phosphorus,and available potassium contents increased significantly,whereas available nitrogen decreased.Sucrase and polyphenol oxidase activities showed stage-specific changes,urease and catalase activities decreased with increasing rain-shelter cultivation duration,and neutral protease activity increased significantly under rain-shelter cultivation.Bacterial community analysis showed that bacterial α-diversity decreased significantly under 6-year rain-shelter cultivation and partially recovered under 12-year rain-shelter cultivation.PCoA analysis indicated significant differences in bacterial community structure among treatments.At the phylum level,Acidobacteria was the dominant group under open-field cultivation,whereas Proteobacteria and Bacteroidetes had higher abundances under rain-shelter cultivation.Correlation analysis indicated that changes in soil pH,salinity,nutrients,and enzyme activities were closely associated with shifts in the abundances of dominant bacterial phyla.Overall,long-term rain-shelter cultivation can drive stage-specific succession of vineyard soil bacterial communities by altering soil pH,salt accumulation,and nutrient status.This study provides a reference for soil health management and sustainable production in rain-shelter vineyards in Shanghai.