两种酿酒葡萄对低温胁迫的生理响应及转录组分析OA

To screen wine grape germplasm with strong cold resistance and clarify their mechanisms of cold resistance,this study used annual branches of Vitis vinifera'Pinot Noir'and'Frontenac'as test materials,establishing two treatments:a control at 4℃and a low-temperature stress at ﹣20℃for 24 hours.The relative electrical conductivity(REC),soluble sugar(SS)content,and peroxidase(POD)activity were determined,and transcriptome sequencing was used to analyze the cold resistance mechanism.The results showed that under low-temperature stress,the REC,SS content,and POD activity of both varieties increased.In the transcriptome analysis,the distribution of upregulated/downregulated differentially expressed genes(601/610)in'Frontenac'was balanced,while the downregulated genes in'Pinot Noir'accounted for a higher proportion(upregulated/downregulated:348/930).GO enrichment analysis revealed that the differentially expressed genes of'Frontenac'were concentrated in phenylpropanoid metabolism,while those of'Pinot Noir'were focused on ethylene response.KEGG enrichment analysis showed that the enriched pathways of both varieties were mainly metabolic pathways,with variety-specific differences.Furthermore,MYB family transcription factors had the highest enrichment level among the differentially expressed genes of the two varieties,and the qRT-PCR verification results of 8 genes were highly consistent with the transcriptome data,confirming the reliability of the sequencing results.This study reveals the differential mechanisms of wine grape varieties differing in cold resistance in response to low-temperature stress at the physiological and molecular levels,providing a theoretical basis for cold-resistant cultivation and management of wine grapes in the Inner Mongolia region.