金寨白叶与福鼎寿眉化学谱及抗慢性溃疡性结肠炎功效比较OA

The characteristic chemical composition and healthy function of Jinzhai Baiye(JZ)and Fuding Shoumei(FD)were unknown.In this study,two white teas were comparatively evaluated in terms of their characteristic chemical compo-sition and anti-DSS induced colitis.The non-volatile chemical constituents between JZ and FD white teas were systemati-cally profiled by using un-targeted metabolomics analysis based on UHPLC-Q-Orbitrap-MS/MS approach.Then,anti-colitis of two white teas was explored based on recently developed DSS-induced chronic colitis model.The results showed that there were significant differences in chemical constituents between JZ and FD white teas.A total of 51 compounds with sig-nificant differences between two white teas were identified,including catechins,flavonoid glycosides,amino acids,pheno-lic acids,lipids.Among them,the contents of some catechins,flavonoid glycosides(rutin,kaempferol 3-O-arabinoglyco-side,quercetin-3-galactoside),amino acids(theanine,arginine)were relatively high in FD.While the contents of some flavonoid glycosides(vitexin,kaempferol,3-(6-acetylgalactoside))and majority of phenolic acids were relatively high in JZ.After 4 cycles of 2%DSS induction,DSS group exhibited chronic syndrome of ulcerative colitis,showing significant decrease in body weight with an obvious undulating trend(P<0.001),shorten colon length,significant increase of DAI scores and splenic index(P<0.001).However,intervention with both JZ and FD significantly improved the syndrome of DSS-induced ulcerative colitis.Interestingly,JZ showed better protective effects than that of FD.Real-Time PCR data revealed that both JZ and FD significantly suppressed the major inflammatory cytokines gene expression,and increased anti-inflammatory cytokine IL-10 expression.JZ proved better anti-inflammatory effects than that of FD.This study provided the characteristic chemical compounds and their anti-inflammatory effects on DSS induced ulcerative colitis between the two white teas,which provided a basis for evaluating the chemical quality and potential health function of JZ white tea.