三叶青地上部分总黄酮的分离纯化、组分分析及其对急性肝损伤小鼠的保护作用OA
Purification,Component Analysis,and Protective Effects of Total Flavonoids from Aerial Parts of Tetrastigma hemsleyanum Diels & Gilg on Acute Liver Injury in Mice
为了优化三叶青地上部分总黄酮(Total Flavonoids from Aerial Parts of Tetrastigma hemsleyanum,TFATH)的大孔树脂纯化工艺,并探讨其对四氯化碳(Carbon Tetrachloride,CCl4)诱导急性肝损伤小鼠的保护作用,采用单因素实验考察不同上样条件和洗脱条件对纯化效果的影响,测定三叶青地上部分样品纯化前后的抗氧化活性,超高效液相串联飞行时间质谱技术(UPLC-ESI-QTOF-MS2)对TFATH进行定性分析,并构建CCl4诱导的急性肝损伤模型.结果显示,纯化后样品黄酮纯度由8.99％增至31.38％,抗氧化能力也显著提高,并从其中检测出了 12种黄酮类物质,主要为(iso)vitexin、(iso)orientin及其糖苷化合物.动物实验结果表明,与模型组相比,TFATH给药组小鼠肝脏指数、血清ALT和AST活性显著降低9.74％、12.91％和33.98％,肝细胞变性、坏死及炎性浸润现象明显改善;肝组织中GSH含量和CAT活性分别提升 54.14％、50.88％,MDA 含量显著降低 66.53％;另外,TFATH 干预可显著下调 TNF-α、IL-1β、IL-6、Caspase-3、Bax和Keapl基因的表达,上调Bcl-2、Nrf2、HO-1、NQO1及GCLC基因的表达.综上所述,TFATH通过激活Keap1/Nrf2信号通路,调节氧化应激,减轻炎症反应,降低促炎因子的表达并抑制肝细胞凋亡,从而全方位地改善CCl4引起的急性肝损伤.该结果为三叶青地上部分资源的开发利用提供理论参考依据.
The aim of this study was to optimize the purification process for total flavonoids from the aerial parts of Tetrastigma hemsleyanum Diels & Gilg(TFATH)by using macroporous resin and to evaluate the protective effects of TFATH against carbon tetrachloride-induced acute liver injury in mice.The effects of different loading conditions and elution conditions on the purification results were investigated using single-factor experiments,and the antioxidant activity of the aerial parts of Tetrastigma hemsleyanum Diels & Gilg was determined before and after purification.TFATH were qualitatively analyzed using UPLC-ESI-QTOF-MS2,and an acute liver injury model induced by carbon tetrachloride was established.The results showed that the purity of flavonoids in the purified samples increased from 8.99％to 31.38％,and the antioxidant capacity also significantly improved.Twelve types of flavonoids were identified,primarily including(iso)vitexin,(iso)orientin,and their glycosides.The animal experiment results showed that,compared with the model group,the liver index and serum ALT and AST activities of TFATH-treated mice significantly decreased by 9.74％,12.91％,and 33.98％,respectively,and the degeneration,necrosis,and inflammatory infiltration of hepatocytes significantly improved.In liver tissue,GSH content and CAT activity increased by 54.14％and 50.88％,respectively,whereas the MDA content significantly decreased by 66.53％.In addition,with the intervention of TFATH,the expression levels of TNF-α,IL-1β,IL-6,caspase-3,Bax,and Keap1 were significantly downregulated,whereas the expression levels of Bcl-2,Nrf2,HO-1,NQO1,and GCLC were upregulated.In summary,TFATH comprehensively ameliorates acute liver injury induced by carbon tetrachloride by activating the Keap 1/Nrf 2 signaling pathway,modulating oxidative stress,alleviating inflammatory responses,reducing the expression of proinflammatory cytokines,and inhibiting hepatocyte apoptosis.The results provide a theoretical reference for the development and utilization of resources from the aerial parts of Tetrastigma hemsleyanum Diels & Gilg.
陈雨薇;毕欣;孙永
南昌大学食品科学与资源挖掘全国重点实验室,江西南昌 330047南昌大学食品科学与资源挖掘全国重点实验室,江西南昌 330047南昌大学食品科学与资源挖掘全国重点实验室,江西南昌 330047
三叶青地上部分总黄酮大孔树脂纯化抗氧化能力组分分析急性肝损伤
total flavonoids from aerial parts of Tetrastigma hemsleyanum Diels & Gilgpurification using macroporous resinantioxidant capacitycomposition analysisacute liver injury
《现代食品科技》 2026 (4)
51-64,14
丽水市农林科学研究院横向课题(HX202107120002)
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