党参、黄芪、茯苓复合饮料的降脂保肝作用研究OA

To investigate the lipid-lowering and hepatoprotective effects and underlying mechanisms of the composite beverage containing Codonopsis pilosula,Astragalus membranaceus,and Poria cocos on hyperlipidemic rats,48 rats were randomly divided into the blank control group,the model group,the simvastatin group,and low-,medium-,and high-dose groups of the composite beverage(518,1036,and 2072 mg/kg,respectively).The composite beverages were gavaged to the rats daily for 8 weeks;the levels of rats blood lipids,serum transaminases,activities of enzymes involved in lipid metabolism,oxidative stress indicators,and inflammatory factors in rats liver tissue were determined using reagent kits;Western blot analysis was used to measure the expression levels of AMPK,Wnt1/β-catenin,and SIRT1 signaling pathway related proteins in rats liver tissue.Compared with the model group,the composite beverage containing Codonopsis pilosula,Astragalus membranaceus,Poria cocos significantly reduced the rats levels of TC,TG,AST,ALT,ACC,GPAT,FAS,MDA,IL-6,and TNF-α(P＜0.01),and significantly increased the rats levels of SOD,GSH-PX,HSL,and LPL(P＜0.01);it also significantly increased the rats protein expression levels of p-AMPK,Wnt1,β-catenin,SIRT1,PGC-1α,and Nrf2(P＜0.01),and significantly decreased the rats protein expression levels of SREBP-1C,HMGCR,C/EBP,PPARγ,and NF-κB(P＜0.01,P＜0.05).In conclusion,the composite beverage containing Codonopsis pilosula,Astragalus membranaceus,and Poria cocos could effectively lower blood lipids and alleviate liver damage in hyperlipidemic rats.Its mechanism was associated with the activation of the AMPK,Wnt1/β-catenin,and SIRT1 signaling pathways,inhibition of fatty acid synthesis,promotion of fatty acid catabolism,suppression of inflammatory responses,and enhancement of antioxidant activity.