高氧-褪黑素协同抑制PLA1β2-PG酶延缓枸杞采后劣变机制OA

A synergistic treatment strategy of high oxygen(80%O2)and melatonin(1.0 mmol/L)was proposed to address the softening and browning issues caused by disrupted cell wall metabolism in fresh goji berries(Lycium barbarum L.)after harvesting.Its regulatory effects and the underlying molecular mechanisms on storage quality of fresh goji berries were systematically analyzed.Fruit firmness and browning index were measured,and PLA1β2 gene expression,polygalacturonase(PG)activity and dynamic changes in cellwall components were analyzed.It was found that synergistic treatment significantly inhibited PLA1β2 gene transcription(downregulated by 55.0%)and reduced PG enzyme activity(decreased by 50.4%),the degradation of covalently bound pectin(CSP)was delayed,and the shelf life of fresh goji berries was extended from 15 days in the control group to 25 days under the combined treatment.Pearson correlation analysis showed a highly significant positive correlation between browning index and PLA1β2 expression level(r=0.89,P＜0.01),indicating their core roles in quality deterioration of fresh goji berries.This study provided a theoretical basis for the development of green preservation technology for fresh goji berries.