枣树寄生粗毛纤孔菌驯化及品质评价OA

A strain was isolated from fruiting body tissue of Inonotus hispidus parasitizing on Ziziphus jujuba.The I.hispidus strain was then cultivated on three kinds of substrates(ZI1,ZI2,ZI3),and the resultant fruiting bodies were measured for hispidin[6-(3,4-dihydroxystyrl)-4-hydroxy-2-pyrone]content and analyzed for high-performance liquid chromatography(HPLC)fingerprint.The results showed that the optimal culture substrate ZI2(64％Z.jujuba wood chips,16％cottonseed hull,19％wheat bran,and 1％quicklime)allowed mycelia to fully colonize the cultivation bags within 80-100 d after inoculation,with fruiting bodies harvested 15-20 d thereafter.The fruiting rate of ZI2 reached 85％,and the yield was(12.87±1.53)g(dry weight).Among the three kinds of substrates,ZI2 also resulted in the highest hispidin content in fruiting bodies,reaching(141.70±1.20)μg·g-1.HPLC fingerprint analysis of the wild and the three groups of cultivated I.hispidus revealed eight common peaks(with hispidin as the reference peak),and the similarity indices were all greater than 0.99.