木蹄层孔菌生物学特性及驯化OA

Wild fruiting bodies of Fomes fomentarius were collected from Betula platyphylla in Saihanba Mechanised Tree Farm,Weichang County,Chengde City,Hebei Province.After tissue isolation,a strain was identified as F.fomentarius,and then the strain was studied for biological characteristics through single-factor experiments and orthogonal experiments.Finally,the strain was domesticated.The results showed that the optimal carbon source,nitrogen source,temperature,and initial pH in single-factor experiments were fructose,yeast extract powder,28 ℃,and pH7,respectively.The optimal conditions determined by the orthogonal experiments were fructose,yeast extract powder,28 ℃,and pH6.After inoculation,the mycelia of the isolated F.fomentarius strain fully colonized the cultivation bags within 50-55 d,and hemispherical primordia formed after an additional 40-45 d.Harvesting was possible after a further 25-35 d of cultivation,with the fruiting bodies measuring 4.5-6.4 cm in diameter and having a base thickness of 2.5-3.0 mm.These findings provided a reference for subsequent development and utilization of F.fomentarius.