草菇海藻糖-6-磷酸合成酶基因克隆鉴定与表达分析OA

Volvariella volvacea is sensitive to low temperature and easy to autolyze,which seriously affects its preservation and circulation on the market.As a critical stress-protective compound,trehalose plays a pivotal role in fungal responses to temperature stress,with trehalose-6-phosphate synthase(TPS)serving as a key enzyme responsible for its biosynthesis.Using V.volvacea strain V34 as the experimental material,the trehalose-6-phosphate synthase(TPS)gene VvTPS in V.volvacea was cloned and then subjected to bioinformatics analyses.The effects of different temperatures(20,25,30,35,and 40 ℃)on VvTPS gene expression and VvTPS enzyme activity were also studied.The results showed that there are the two TPS genes in V.volvacea,namely VvTPS1 and VvTPS2,which encode 882 and 1018 amino acids,respectively.The promoter regions of the two VvTPS contain various ciss-acting elements responsive to hormones and stress conditions.Both VvTPSs are localized in the cytoplasm and contain a typical HAD conserved domain.Within the experimental temperature range,both VvTPS1 and VvTPS2 reached the highest expression at 20 ℃.Their expression levels were relatively high at 40 ℃ and were lowest at 35 ℃.The VvTPS activity in V.volvacea was the highest at 25 ℃ and lowest at 35 ℃.These findings provided a reference for stress-resistant breeding of V.volvacea.