香菇降解柠条基质关键基因挖掘OA

Caragana korshinskii wood chips were used in replacement of traditional mixed wood chips(CK)at different proportions(10％,20％,30％)in Lentinula edodes culture substrate.The effects of the amount of C.korshinskii wood chips in the substrate were investigated on the growth,development,and nutritional components of L.edodes.Weighted gene co-expression network analysis(WGCNA)was used to identify functional genes associated with different developmental stages and to screen for core genes involved in lignocellulose degradation,thereby providing a reference for elucidating the molecular mechanisms underlying degradation of C.korshinskii by L.edodes.The results showed that the 30％C.korshinskii(N3)group achieved the highest yield,reaching 70.62 g per cultivation bag,which was 41.2％higher than that of the CK.The N3 group also resulted in the highest protein and ash contents in the fruiting bodies,while the 20％C.korshinskii(N2)group had the lowest fat content,and the 10％C.korshinskii(N1)group showed the highest total amino acid content.Five gene modules closely related to the growth of L.edodes were identified by WGCNA:the Red module(1 257 genes)was positively correlated with the browning stage;the Brown module(1 543 genes)was negatively correlated with the browning stage;the Greenyellow module(136 genes)and Blue module(2 062)were positively correlated with the fruiting body stage;and the Turquoise module(6 132 genes)was negatively correlated with the fruiting body stage.Functional gene enrichment analysis revealed significant metabolic pathways such as pyruvate metabolism,arginine and proline metabolism,and the citric acid cycle during the browning stage,while metabolic pathways such as starch and sucrose metabolism,penicillin and cephalosporin biosynthesis,and other glycan degradation were significantly enriched during the fruiting body stage.Two genes potentially involved in lignocellulose degradation,CYP450 and GH,were selected,along with 18 genes(ACPT,2-NPD,PTP1B,HLYD,VPS14C,MetE,etc.)possibly closely related to the growth and development of L.edodes.These findings provided a reference for in-depth study of the molecular mechanisms of lignocellulose degradation in L.edodes.