天然深共晶溶剂联合微波提取柚子皮总黄酮及其体外抗氧化活性分析OA
Extraction process optimization and antioxidant activity of total flavonoids from pomelo peel by natural deep eutectic solvents combined with microwaves
[目的]以通过天然深共晶溶剂联合微波技术优化柚子皮总黄酮提取工艺.[方法]分析 3种天然深共晶溶剂(NADES)和传统提取剂联合微波提取柚子皮黄酮的能力,通过响应面法优化其提取工艺条件,并评估柚子皮提取物的抗氧化活性.[结果]由氯化胆碱、苹果酸、乙二醇组成的NADES在液料比23∶1(mL/g),加水量29%,提取温度73℃,微波时间40 s下具有最佳的总黄酮得率,为2.20%.最优工艺条件下,柚子皮总黄酮的DPPH自由基清除率为93.32%、羟自由基清除率为87.15%、还原力为0.57、金属离子螯合率为54.92%、脂质过氧化抑制率为29.78%.[结论]天然深共晶溶剂联合微波提取柚子皮的黄酮提取率较高,且黄酮体外抗氧化活性较好.
[Objective]To optimize the extraction process of total flavonoids from pomelo peel by natural deep eutectic solvents(NADES)combined with microwaves.[Methods]The extraction ability of flavonoids from pomelo peel is analyzed among three NADES and conventional extractants combined with microwaves.Additionally,the extraction process is optimized by response surface experiment,and the antioxidant activity of the extracts is evaluated.[Results]The optimal total flavonoid extraction rate is 2.20 percent at a liquid-solid ratio of 23∶1(mL/g),a water content of 29%,an extraction temperature of 73℃,and a microwave time of 40 seconds.Under these conditions,the DPPH and hydroxyl radical scavenging rate are 93.32%and 87.15%,the reducing power is 0.57,the metal ion chelation rate is 54.92%,and the lipid peroxidation inhibition rate is 29.78%.[Conclusion]A high extraction rate of flavonoids from pomelo peel is achieved by NADES combined with microwaves,with the resulting flavonoids exhibiting strong in vitro antioxidant activity.
谢文;张硕;王芳;黄昕懿
成都师范学院化学与生命科学学院,四川 成都 611130成都师范学院化学与生命科学学院,四川 成都 611130||特色园艺生物资源开发与利用四川省高等学校重点实验室,四川 成都 611130成都师范学院化学与生命科学学院,四川 成都 611130成都师范学院化学与生命科学学院,四川 成都 611130
柚子皮总黄酮天然深共晶溶剂微波辅助抗氧化性
pomelo peeltotal flavonoidsnatural deep eutectic solvent(NADES)microwave assistedantioxidant activity
《食品与机械》 2026 (2)
198-205,8
教育部大学生创新创业训练支持项目(编号:S202414389072)四川省自然科学基金(面上)(编号:2024NSFSC0370)
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