微量元素对马瑟兰葡萄果实糖酸代谢及基因表达的影响OA

Sugar-acid metabolism critically influences grape berry quality,with trace elements serving as key modulators of this process.In this study,'Marselan'grapevines were foliar-sprayed with a trace element mixture(Mn,Zn,Cu,Mo,B)from flowering to veraison.The experiment included a water control group(CK)and treatment group with mixtures of trace elements at three concentra-tions.We measured sugar and acid components,trace element content,key metabolic enzyme activities,and the expression of acid me-tabolism-related genes at different growth stages in grape.These analyses aimed to evaluate the influence of trace elements on grape quali-ty.Foliar application of trace elements significantly increased the contents of total soluble sugar(TSS),glucose(Glu),sucrose(Sue),and fructose(Fru)in grape.The treatments also enhanced the activities of sucrose synthase synthetic direction(SS-ss)and sucrose phos-phate synthase(SPS).Concurrently,the concentrations of tartaric acid(TA),malic acid(MA),oxalic acid(OA),and citric acid(CA)decreased.The activities of nicotinamide adenine dinucleotide phosphate-malic enzyme(NADP-ME)and citrate synthase(CS)were elevated.In contrast.The activities of nicotinamide adenine dinucleotide-malic dehydrogenase(NAD-MDH),nicotinamide adenine dinucleotide-isocitrate dehydrogenase(NAD-IDH),phosphoenolpyruvate carboxylase(PEPC),cytoplasmic aconitase(Cyt-ACO),and mitochondrial aconitase(Mit-ACO)were inhibited.Furthermore,zinc accumulation was promoted in the berries.The expression of the phosphoenol pyruvate carboxylase gene was significantly up-regulated.In summary,the T1 treatment(0.025 g/L Mn,0.04 g/L Zn,0.016 g/L Cu,0.025 g/L Mo,0.075 g/L B)was the most effective.Foliar application of trace elements can regulate the activity of key enzymes and gene expression,effectively promoting sugar accumulation and acid metabolism,thereby improving fruit quality.