蛹虫草活性多肽的制备及其神经保护作用探究OA

Cordyceps militaris is a high-quality functional food rich with various bioactive substances.This study aimed to extract pro-teins from C.militaris and prepare bioactive peptides through specific enzymatic hydrolysis,followed by separation,purification,and iden-tification.The neuroprotective effects of these bioactive peptides were investigated.Results showed that hydrolysates derived from C.milita-ris proteins through simulated gastrointestinal digestion exhibited significant neuroprotective activity.Ultrafiltration was used to fractionate the hydrolysates,and the hydrolysate fraction with＜3 kDa demonstrated the strongest neuroprotective effects.Further analysis identified 76 small peptides within this fraction as the main contributors to its bioactivity,which was closely associated with the high content of hy-drophobic amino acids and aromatic amino acids.Among these peptides,a specific peptide monomer,NWVPGD,was identified.NWVPGD effectively mitigated glutamate-induced damage in PC12 cells,exhibited significant free radical scavenging activity,and demon-strated the potential to activate the Keap1-Nrf2 antioxidant pathway.Its antioxidant properties likely played a critical role in its neuropro-tective effects.This study successfully identified and characterized a well-defined bioactive peptide from C.militaris,providing support for the development of C.militaris-derived peptides with neuroprotective functions.